The first 100K reads are subsetted from these samples from GEO accesssion GSE91064: 
BY4741_rep3 (wild type), 
hyp2-1_rep1 (eIF5A mutant tif51A-1), 

Citation: 

Pelechano V, Alepuz P. eIF5A facilitates translation termination globally and promotes the elongation of many non polyproline-specific tripeptide sequences. Nucleic Acids Res 2017;45:7326

The folder contains Saccharomyces_cerevisiae.R64-1-1 genome fasta and gff files from Ensembl. 

Preprocessing with the script src/preprocess_test.sh will do the following: 
- adapter trimming with cutadapt (standard illumina adapters)
- UMI extraction
- Mapping to Saccharomyces_cerevisiae.R64-1-1 genome with STAR 
- UMI deduplication
- BEDGRAPH generation